1WU9
Crystal structure of the C-terminal domain of the end-binding protein 1 (EB1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.000, 37.300, 56.500 |
| Unit cell angles | 90.00, 105.40, 90.00 |
Refinement procedure
| Resolution | 54.230 - 1.540 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.177 |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 1.600 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Number of reflections | 18705 | |
| <I/σ(I)> | 14.8 | 5.4 |
| Completeness [%] | 97.4 | 90.1 |
| Redundancy | 2.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | sodium citrate, PEG 3350, gamma-butyrolactone, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
