1WU6

Crystal structure of reducing-end-xylose releasing exo-oligoxylanase E70A mutant complexed with xylobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-30
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	53.300, 86.607, 87.711
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.390 - 1.450
R-factor	0.171
Rwork	0.171
R-free	0.18000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h14
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.020	1.500
High resolution limit [Å]	1.450	1.450
Number of reflections	72191
<I/σ(I)>	26.5	3.9
Completeness [%]	99.2	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	PEG4000, sodium acetate, glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K