1WU2
Crystal Structure of molybdopterin biosynthesis moeA protein from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-08 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 0.97904, 0.97939, 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.149, 65.912, 76.684 |
| Unit cell angles | 90.00, 102.17, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.23574 |
| Rwork | 0.233 |
| R-free | 0.28226 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.943 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.072 | |
| Number of reflections | 35136 | |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 98.9 | 98 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbacth | 7.6 | 295 | PEG 4k, HEPES, pH 7.6, microbacth, temperature 295K |
