1WTX

Hyperthermophile chromosomal protein SAC7D single mutant V26A in complex with DNA GTAATTAC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	150
Detector technology	IMAGE PLATE
Collection date	2001-01-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	36.113, 50.443, 78.153
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.890 - 2.200
R-factor	0.2253
Rwork	0.224
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1azq
RMSD bond length	0.016
RMSD bond angle	1.580
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.042	0.403
Number of reflections	7491
<I/σ(I)>	17	2.9
Completeness [%]	97.1	88.7
Redundancy	4.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	PEG 400, Tris buffer, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 400
2	1	1	Tris buffer
3	1	2	PEG 400
4	1	2	Tris buffer