1WTV
Hyperthermophile chromosomal protein SAC7D single mutant M29A in complex with DNA GTAATTAC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL17B2 |
Synchrotron site | NSRRC |
Beamline | BL17B2 |
Temperature [K] | 150 |
Detector technology | IMAGE PLATE |
Collection date | 2002-11-29 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.11634 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.923, 47.968, 77.777 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.600 |
R-factor | 0.2132 |
Rwork | 0.210 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1azq |
RMSD bond length | 0.017 |
RMSD bond angle | 1.790 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.049 | 0.490 |
Number of reflections | 17958 | |
<I/σ(I)> | 24 | 2.8 |
Completeness [%] | 98.0 | 96.8 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | PEG 400, Tris buffer , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 400 | ||
2 | 1 | 1 | Tris buffer | ||
3 | 1 | 2 | PEG 400 | ||
4 | 1 | 2 | Tris buffer |