1WTQ
Hyperthermophile chromosomal protein SAC7D single mutant M29F in complex with DNA GTAATTAC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 150 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-09-20 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.237, 50.053, 35.772 |
| Unit cell angles | 90.00, 108.96, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.700 |
| R-factor | 0.181 |
| Rwork | 0.176 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1azq |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.830 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.027 | 0.068 |
| Number of reflections | 11165 | |
| <I/σ(I)> | 55.6 | 14.9 |
| Completeness [%] | 94.4 | 85.4 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | PEG 400, Tris buffer, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | Tris buffer | ||
| 3 | 1 | 2 | PEG 400 | ||
| 4 | 1 | 2 | Tris buffer |
