1WTP

Hyperthermophile chromosomal protein SAC7D single mutant M29F in complex with DNA GCGA(UBr)CGC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	150
Detector technology	IMAGE PLATE
Collection date	2002-09-16
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	38.217, 47.889, 52.494
Unit cell angles	90.00, 102.67, 90.00

Refinement procedure

Resolution	40.000 - 1.900
R-factor	0.228
Rwork	0.222
R-free	0.28720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1azp
RMSD bond length	0.015
RMSD bond angle	1.660
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.049	0.269
Number of reflections	14849
<I/σ(I)>	26.4	4
Completeness [%]	98.6	93.4
Redundancy	3.7	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	PEG 400, Tris buffer, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 400
2	1	1	Tris buffer
3	1	2	PEG 400
4	1	2	Tris buffer