1WTJ

Crystal Structure of delta1-piperideine-2-carboxylate reductase from Pseudomonas syringae pvar.tomato

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-31
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	67.443, 68.968, 178.952
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.500 - 1.550
R-factor	0.194
Rwork	0.194
R-free	0.21500
Structure solution method	MIR
RMSD bond length	0.004
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.529	1.610
High resolution limit [Å]	1.550	1.550
Number of reflections	119237
Completeness [%]	97.8	98.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Na/K Tartrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K