1WTH

Crystal structure of gp5-S351L mutant and gp27 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-15
Detector	SBC
Wavelength(s)	1.03320
Spacegroup name	H 3 2
Unit cell lengths	137.134, 137.134, 396.042
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.550 - 2.800
R-factor	0.217
Rwork	0.217
R-free	0.28100
Structure solution method	MIR
Starting model (for MR)	1k28
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.550	2.930
High resolution limit [Å]	2.800	2.800
Number of reflections	33875
Completeness [%]	94.7	87.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	279	PEG 8000, Tris, Glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K