1WSC

Crystal structure of ST0229, function unknown protein from Sulfolobus tokodaii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL40B2
Synchrotron site	SPring-8
Beamline	BL40B2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9792, 0.9794, 0.9000
Spacegroup name	C 2 2 21
Unit cell lengths	69.863, 113.657, 111.623
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.990 - 2.450
R-factor	0.217
Rwork	0.217
R-free	0.27800
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.450
Number of reflections	16391

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	PEG4000, isopropanol, citrate, gamma-butyllactone, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K