1WRZ
Calmodulin complexed with a peptide from a human death-associated protein kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8043 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.070, 33.760, 73.870 |
| Unit cell angles | 90.00, 110.04, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.22711 |
| Rwork | 0.224 |
| R-free | 0.27872 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.988 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 10397 | |
| <I/σ(I)> | 11.1 | 3.5 |
| Completeness [%] | 98.0 | 99 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 295 | sodium acetate, PEG 1500, calcium chloride, pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
