1WRR
Urate oxidase from aspergillus flavus complexed with 5-amino 6-nitro uracil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.972 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.385, 96.469, 106.131 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.000 - 1.640 |
| R-factor | 0.1697 |
| Rwork | 0.168 |
| R-free | 0.18518 |
| Structure solution method | SIR under pressure of noble gaz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.407 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.680 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Number of reflections | 48554 | |
| <I/σ(I)> | 17.8 | 10.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 8.5MG/ML PROTEIN, 0.2MG/ML DIAMINOURACIL, 5-7%(W/V) PEG 8000, 100MM TRIS/HCL, pH 8.00, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
