1WRD

Crystal structure of Tom1 GAT domain in complex with ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9780
Spacegroup name	F 4 3 2
Unit cell lengths	168.317, 168.317, 168.317
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.450 - 1.750
R-factor	0.214
Rwork	0.213
R-free	0.25900
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	1.031
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.057	0.421
Number of reflections	20997
<I/σ(I)>	20.9	8.2
Completeness [%]	99.5	99.4
Redundancy	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	sodium citrate, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	sodium citrate, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	sodium citrate, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K