1WQW
Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii Ot3 in complex with Biotinyl-5-AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-06-27 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.348, 82.832, 72.785 |
Unit cell angles | 90.00, 102.15, 90.00 |
Refinement procedure
Resolution | 37.490 - 1.450 |
Rwork | 0.212 |
R-free | 0.24000 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1wq7 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.052 | 0.375 |
Number of reflections | 71511 | |
<I/σ(I)> | 12 | 3.57 |
Completeness [%] | 94.3 | 96.3 |
Redundancy | 3.5 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 295 | PEG20K, Acetate, NaOH, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |