1WQS

Crystal structure of Norovirus 3C-like protease

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-01
Detector	MARRESEARCH
Wavelength(s)	1.0050, 1.0091, 1.0013, 0.9800
Spacegroup name	P 61
Unit cell lengths	128.890, 128.890, 118.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.206
Rwork	0.206
R-free	0.24000
Structure solution method	MAD
RMSD bond length	0.052
RMSD bond angle	4.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.530	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.088	0.438
<I/σ(I)>	8.8	2.5
Completeness [%]	99.8	100
Redundancy	2.76	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	POTASSIUM SODIUM TARTRATE TETRAHYDRATE, SODIUM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K