1WQ1
RAS-RASGAP COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-18 |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-03-14 |
| Detector | SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.900, 41.100, 89.000 |
| Unit cell angles | 90.00, 108.40, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.31900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RAS:GPPNP (PDB ENTRY 5P21) GAP-334 (PDB ENTRY 1WER) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.700 * |
| Data reduction software | XDS ((W.KABSCH)) |
| Data scaling software | XDS ((W. KABSCH)) |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.066 * | |
| Total number of observations | 35339 * | |
| Number of reflections | 15811 | |
| <I/σ(I)> | 10 | 1.1 |
| Completeness [%] | 89.0 | 64 |
| Redundancy | 2.2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | drop | 20 (mM) | ||
| 5 | 1 | reservoir | PEG3350 | 15-20 (%) | |
| 6 | 1 | reservoir | HEPES | 100 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 20 (mM) | |
| 8 | 1 | reservoir | 20 (mM) |
