1WPY
Crystal Structure Of Biotin-(Acetyl-CoA-Carboxylase) ligase From Pyrococcus Horikoshii Ot3 in complex with biotin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-17 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.9650 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.420, 82.737, 72.711 |
| Unit cell angles | 90.00, 103.17, 90.00 |
Refinement procedure
| Resolution | 33.560 - 1.600 |
| Rwork | 0.194 |
| R-free | 0.21300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1wnl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.560 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.258 |
| Number of reflections | 56262 | |
| <I/σ(I)> | 21.4 | 4.2 |
| Completeness [%] | 96.4 | 96 |
| Redundancy | 3.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 295 | PEG20K, Acetate, NaOH, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
