1WPW
Crystal Structure of IPMDH from Sulfolobus tokodaii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 67.910, 91.500, 132.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.730 - 2.800 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.280 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 20835 | |
| Completeness [%] | 98.6 | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | ammonium sulfate, MPD, magnesium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
