1WPT
Crystal Structure of HutP, an RNA binding anti-termination protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2003-10-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9780 |
Spacegroup name | P 21 3 |
Unit cell lengths | 95.410, 95.410, 95.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.480 - 2.700 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vea |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.041 | 0.350 |
Number of reflections | 8514 | |
Completeness [%] | 93.1 | 100 |
Redundancy | 12.2 | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | PEG2K, MME, HEPES, MgCl2, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |