1WP5
Crystal structure of the C-terminal domain of DNA topoisomerase IV
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.95370, 0.97994, 0.97966 |
| Spacegroup name | P 32 |
| Unit cell lengths | 83.211, 83.211, 44.999 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.000 - 1.790 |
| R-factor | 0.16262 |
| Rwork | 0.160 |
| R-free | 0.21381 |
| Structure solution method | MAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.773 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.300 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.800 |
| Number of reflections | 30204 | |
| Completeness [%] | 92.9 | 70.3 |
| Redundancy | 5.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | magnesium acetate, sodium cacodylate, MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
