1WP5
Crystal structure of the C-terminal domain of DNA topoisomerase IV
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.95370, 0.97994, 0.97966 |
Spacegroup name | P 32 |
Unit cell lengths | 83.211, 83.211, 44.999 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.000 - 1.790 |
R-factor | 0.16262 |
Rwork | 0.160 |
R-free | 0.21381 |
Structure solution method | MAD |
RMSD bond length | 0.024 |
RMSD bond angle | 1.773 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.300 | 1.850 |
High resolution limit [Å] | 1.790 | 1.800 |
Number of reflections | 30204 | |
Completeness [%] | 92.9 | 70.3 |
Redundancy | 5.3 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | magnesium acetate, sodium cacodylate, MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |