1WP1
Crystal structure of the drug-discharge outer membrane protein, OprM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2003-05-30 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 85.430, 85.430, 1044.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.630 - 2.560 |
| R-factor | 0.25503 |
| Rwork | 0.252 |
| R-free | 0.30772 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.386 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.700 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.047 | 0.438 |
| Number of reflections | 48709 | |
| <I/σ(I)> | 6.4 | 2 |
| Completeness [%] | 99.1 | 94.5 |
| Redundancy | 11.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | sodium acetate, imidazole, octyl-POE, CYMAL-3, MPD, tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
