1WOZ
Crystal structure of uncharacterized protein ST1454 from Sulfolobus tokodaii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97967, 0.97997, 0.9000 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 84.670, 84.670, 84.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.940 |
| Rwork | 0.174 |
| R-free | 0.20700 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.505 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 15276 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.2 | 19.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 293 | HEPES buffer, Ammonium sulfate, PEG400, Polypropylene glycol 400, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
