1WOY

Crystal structure of methionyl tRNA synthetase Y225F mutant from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-03
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	64.416, 82.731, 141.405
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.189
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 1A8H
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	47006
<I/σ(I)>	15.6
Completeness [%]	90.3	72.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	2-propanol, sodium citrate, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K