1WOW
Crystal structure of heme oxygenase-2 from Synechocystis sp. PCC 6803 complexed with heme in ferrous form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.046 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.030, 74.280, 72.270 |
| Unit cell angles | 90.00, 108.03, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wov |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.139 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 29153 | |
| Completeness [%] | 97.0 | 93.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | pottasium sodium tartrate, pottasium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
