1WOU

Crystal Structure of human Trp14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	2001-02-21
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	26.860, 48.390, 81.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.570 - 1.800
R-factor	0.219
Rwork	0.219
R-free	0.22900
Structure solution method	MAD
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	CNS (0.9)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.800
Rmerge	0.041
Number of reflections	10385
Completeness [%]	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	polyethylene glycol 8000, polyethylene glycol monomethylether 2000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	polyethylene glycol 8000, polyethylene glycol monomethylether 2000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K