1WOC
Crystal structure of PriB
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97137, 0.9790, 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.521, 70.317, 74.805 |
| Unit cell angles | 90.00, 131.59, 90.00 |
Refinement procedure
| Resolution | 9.000 - 2.000 |
| Rwork | 0.221 |
| R-free | 0.27620 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.521 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | 0.112 |
| Number of reflections | 27253 | |
| <I/σ(I)> | 22.6 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 25%(v/v) MPD, 50mM Magnesium Chloride, 100mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
