1WNI

Crystal Structure of H2-Proteinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	293
Detector technology	DIFFRACTOMETER
Detector	WEISSENBERG
Wavelength(s)	1.07
Spacegroup name	P 43 21 2
Unit cell lengths	77.800, 77.800, 82.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.176
Structure solution method	MIR
Data reduction software	WEIS
Data scaling software	WEIS
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
High resolution limit [Å]	2.200
Rmerge	0.069
Number of reflections	14262
Completeness [%]	86.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	AMMONIUM SULFATE, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K