1WNH
Crystal structure of mouse Latexin (tissue carboxypeptidase inhibitor)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0781, 0.9794, 0.9796, 0.9077 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 103.900, 103.900, 60.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.470 - 1.830 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.23700 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.039 | 0.422 |
Number of reflections | 29633 | |
<I/σ(I)> | 19.9 | 4.5 |
Completeness [%] | 99.7 | 100 |
Redundancy | 14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.8M ammonium sulfate, 0.1M cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |