1WNE
Foot and Mouth Disease Virus RNA-dependent RNA polymerase in complex with a template-primer RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9340 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 94.627, 94.627, 100.513 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.520 - 3.000 |
| R-factor | 0.273 |
| Rwork | 0.273 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u09 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.071 |
| Number of reflections | 10479 |
| <I/σ(I)> | 11.7 |
| Completeness [%] | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 4000, magnesium acetate, sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | magnesium acetate | ||
| 3 | 1 | 1 | sodium cacodylate | ||
| 4 | 1 | 2 | PEG 4000 | ||
| 5 | 1 | 2 | magnesium acetate |
