1WN7

Crystal structure of archaeal family B DNA polymerase mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-17
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	74.344, 111.131, 111.559
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.260 - 2.750
R-factor	0.226
Rwork	0.226
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1GCX
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.260	2.920
High resolution limit [Å]	2.750	2.750
Number of reflections	22320
Completeness [%]	90.4	79.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG 1450, NiCl2, glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K