1WN2
Crystal structure of project ID PH1539 from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-19 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 49.670, 49.670, 85.791 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.120 - 1.200 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rlk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.055 | 0.336 |
| Number of reflections | 34325 | |
| <I/σ(I)> | 15.4 | 4.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.98 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbach | 9.2 | 291 | PEG 20k 16.5w/v(%), Ches 0.1M, pH 9.2, Microbach, temperature 291K |
