1WMZ
Crystal Structure of C-type Lectin CEL-I complexed with N-acetyl-D-galactosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-10 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.580, 52.240, 136.590 |
| Unit cell angles | 90.00, 91.66, 90.00 |
Refinement procedure
| Resolution | 33.810 - 1.700 |
| R-factor | 0.15958 |
| Rwork | 0.158 |
| R-free | 0.18644 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.605 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 141.400 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 59176 | |
| Completeness [%] | 96.3 | 84.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 60% 2-methyl-2,4-pentanediol, 1mM N-acetyl-D-galactosamine, 10mM calcium chloride, 0.1M Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
