1WMX
Crystal Structure of Family 30 Carbohydrate Binding Module
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-03 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.720, 81.860, 86.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.290 - 2.000 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.23300 |
| Structure solution method | SIR |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.720 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.042 | 0.319 |
| Number of reflections | 28642 | |
| Completeness [%] | 86.8 | 70.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 288 | 100mM Tris buffer(pH7.5), 10mM Nickel Chloride, 1.0M Lithium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
