1WMU
Crystal Structure of Hemoglobin D from the Aldabra Giant Tortoise, Geochelone gigantea, at 1.65 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-24 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.244, 62.138, 53.926 |
| Unit cell angles | 90.00, 109.98, 90.00 |
Refinement procedure
| Resolution | 40.480 - 1.650 |
| Rwork | 0.193 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v75 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNX (2000.1) |
| Refinement software | CNX (2000.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.480 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.031 | 0.211 |
| Number of reflections | 39802 | |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 94.7 | 90.6 |
| Redundancy | 7.39 | 7.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 50mM Tris-HCl, pH 7.5, temperature 293K |
