1WMK
Human death-associated kinase DRP-1, mutant S308D d40
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.190, 143.290, 255.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.600 |
| R-factor | 0.2773 |
| Rwork | 0.276 |
| R-free | 0.29577 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SXO |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.874 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.700 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Number of reflections | 37261 | |
| <I/σ(I)> | 7.6 | 3.6 |
| Completeness [%] | 99.2 | 99.9 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 295 | PEG, sodium acetate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
