1WME
Crystal Structure of alkaline serine protease KP-43 from Bacillus sp. KSM-KP43 (1.50 angstrom, 293 K)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2000-10-29 |
| Detector | ADSC QUAMTUM 4r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 43.539, 110.386, 168.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.000 - 1.500 |
| R-factor | 0.126 |
| R-free | 0.16580 |
| Structure solution method | MIR |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.028 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.061 | 0.277 |
| Number of reflections | 63023 | |
| <I/σ(I)> | 15.712 | 3.041 |
| Completeness [%] | 96.0 | 59.2 |
| Redundancy | 4.063 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | 0.1M HEPES-NaOH pH 7.3, 1.7M ammonium sulfate, 10mM calcium chloride, 10% (v/v) 1,4-dioxane, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
