1WM6
Crystal structure of TT0310 protein from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45PX |
| Synchrotron site | SPring-8 |
| Beamline | BL45PX |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-07-04 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.97925 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 185.632, 206.252, 107.977 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.400 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.084 | 0.538 |
| Number of reflections | 80972 | |
| <I/σ(I)> | 10.2 | 3.6 |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 6.5 | 5.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.5 | 18 * | Sodium acetate trihydrate, sodium cacodylate, pH 6.5, Microbatch, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 30.0 (mg/ml) | |
| 2 | 1 | 1 | 50 (mM) | ||
| 3 | 1 | 1 | Tris-HCl | 20 (mM) | pH8.0 |
| 4 | 1 | 1 | sodium acetate | 1.4 (M) | |
| 5 | 1 | 1 | cacodylate-NaOH | 0.1 (M) | pH6.5 |
