1WM5
Crystal structure of the N-terminal TPR domain (1-203) of p67phox
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 121.671, 121.671, 45.709 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.420 - 1.950 |
| R-factor | 0.189 |
| Rwork | 0.185 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e96 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.900 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.058 | 0.189 |
| Number of reflections | 17115 | |
| <I/σ(I)> | 8 | |
| Completeness [%] | 93.1 | 60.4 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 277 | ammonium sulfate, HEPES, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
