1WLR
Apo aminopeptidase P from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200H |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-08 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 177.322, 177.322, 96.312 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.15036 |
| Rwork | 0.149 |
| R-free | 0.17997 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | Native aminopeptidase P without water Mn or multiple conformers |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.290 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 47221 | |
| <I/σ(I)> | 32.93 | |
| Completeness [%] | 90.2 | 59.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 298 | TRIS, PEG 4000, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
