1WLD

Congerin II T88I single mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-09-17
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 42 21 2
Unit cell lengths	60.970, 60.970, 79.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.600
Rwork	0.187
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.600
Rmerge	0.049
Number of reflections	19825
Completeness [%]	96.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.8M magnesium sulfate, 0.93mM lactose, 0.05M Mes, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K