1WL6

Mg-substituted form of E. coli aminopeptidase P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	1998-08-24
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 64 2 2
Unit cell lengths	177.733, 177.733, 96.484
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.260 - 2.000
R-factor	0.14832
Rwork	0.148
R-free	0.17336
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	NATIVE AMINOPEPTIDASE P WITHOUT HETATOMS
RMSD bond length	0.011
RMSD bond angle	1.296
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.260	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	56174
<I/σ(I)>	26.53	5.39
Completeness [%]	92.4	64.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			TRIS, PEG 4000, VAPOR DIFFUSION, HANGING DROP