1WKR
Crystal structure of aspartic proteinase from Irpex lacteus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2001-05-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.270, 78.898, 54.072 |
| Unit cell angles | 90.00, 96.71, 90.00 |
Refinement procedure
| Resolution | 26.990 - 1.300 |
| R-factor | 0.147 |
| Rwork | 0.147 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pep |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.037 | 0.222 |
| Number of reflections | 75525 | |
| <I/σ(I)> | 25.4 | 4.9 |
| Completeness [%] | 99.1 | 98.3 |
| Redundancy | 5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 50% saturated ammonium phosphate, 10mM sodium citrate-sulfate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
