1WKP
Flowering locus t (ft) from arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.973, 61.542, 66.841 |
| Unit cell angles | 75.15, 72.18, 70.55 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.18358 |
| Rwork | 0.181 |
| R-free | 0.22611 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | arabidopsis thaliana terminal flower 1 (tfl1) |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.076 | 0.170 |
| Number of reflections | 21870 | |
| <I/σ(I)> | 12.05 | 3.8 |
| Completeness [%] | 93.2 | 66.2 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 291 | 0.14M Ammonium Sulfate, 38% w/v PEG 5000 MME, 0.1M MES, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
