1WKF
TRNA-GUANINE TRANSGLYCOSYLASE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07-14 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.400, 64.300, 72.000 |
| Unit cell angles | 90.00, 97.20, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.200 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.25000 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.642 |
| Data reduction software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.200 * |
| Rmerge | 0.038 |
| Number of reflections | 19632 |
| Completeness [%] | 93.7 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 25 * | Romier, C., (1996) Proteins: Struct.,Funct., Genet., 24, 516. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | PEG8000 | 6.5 (%) | |
| 4 | 1 | drop | DTT | 0.5 (mM) | |
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 13 (%) | |
| 7 | 1 | reservoir | DTT | 1 (mM) |
