1WKD

TRNA-GUANINE TRANSGLYCOSYLASE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1996-07-10
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	90.700, 63.700, 72.500
Unit cell angles	90.00, 96.40, 90.00

Refinement procedure

Resolution	6.000 - 2.600
R-factor	0.204
Rwork	0.204
R-free	0.27600
RMSD bond length	0.015
RMSD bond angle	1.738
Data reduction software	XDS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.600 *
Rmerge	0.065
Number of reflections	12629
Completeness [%]	98.7
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		25 *	Romier, C., (1996) Proteins: Struct.,Funct., Genet., 24, 516. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop	PEG8000	6.5 (%)
4	1	drop	DTT	0.5 (mM)
5	1	reservoir	Tris-HCl	100 (mM)
6	1	reservoir	PEG8000	13 (%)
7	1	reservoir	DTT	1 (mM)