1WK2

Crystal structure of a hypothetical protein from thermus thermophilus HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-17
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.97913, 0.9, 0.97942
Spacegroup name	C 1 2 1
Unit cell lengths	52.583, 55.846, 43.186
Unit cell angles	90.00, 96.60, 90.00

Refinement procedure

Resolution	29.780 - 2.500
R-factor	0.344
Rwork	0.327
R-free	0.38900
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	43.000
High resolution limit [Å]	1.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	23% PEG3350, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	23% PEG3350, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	23% PEG3350, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	23% PEG3350, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K