1WK2
Crystal structure of a hypothetical protein from thermus thermophilus HB8
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-17 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.97913, 0.9, 0.97942 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 52.583, 55.846, 43.186 |
Unit cell angles | 90.00, 96.60, 90.00 |
Refinement procedure
Resolution | 29.780 - 2.500 |
R-factor | 0.344 |
Rwork | 0.327 |
R-free | 0.38900 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.000 |
High resolution limit [Å] | 1.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 23% PEG3350, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 23% PEG3350, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 23% PEG3350, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 23% PEG3350, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |