1WIW

Crystal structure of a glucose-6-phosphate isomerase like protein from thermus thermophilus HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-18
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.97902, 0.90000, 0.97932
Spacegroup name	P 21 21 21
Unit cell lengths	51.405, 92.075, 116.079
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.480 - 2.000
R-factor	0.23
Rwork	0.195
R-free	0.23500
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.840	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.088
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	23% PEG3350, 0.2M MgCl2, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K