1WHZ
Crystal structure of a hypothetical protein from thermus thermophilus HB8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-13 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9000, 0.979105, 0.979416 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.040, 27.910, 36.990 |
| Unit cell angles | 90.00, 90.31, 90.00 |
Refinement procedure
| Resolution | 36.990 - 1.520 |
| R-factor | 0.148 |
| Rwork | 0.148 |
| R-free | 0.20900 |
| Structure solution method | MAD |
| RMSD bond length | 0.035 |
| RMSD bond angle | 2.800 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.990 | 1.620 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Completeness [%] | 99.6 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9.2 | 293 | PegMME, natrium chloride, Bicine, pH 9.2, VAPOR DIFFUSION, temperature 293K |
