1WHS

STRUCTURE OF THE COMPLEX OF L-BENZYLSUCCINATE WITH WHEAT SERINE CARBOXYPEPTIDASE II AT 2.0 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	95.600, 95.600, 208.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300 *
R-factor	0.175
RMSD bond length	0.012
RMSD bond angle	2.300
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.048 *
Total number of observations	52849 *
Number of reflections	29338 *
Completeness [%]	72.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2-3 (mg/ml)
2	1	drop	sodium chloride	0.25 (M)
3	1	drop	PEG1550	1-4 (%)
4	1	drop	sodium acetate	40 (mM)
5	1	reservoir		1.5-2.0 (M)
6	1	reservoir	sodium acetate	40 (mM)