1WGC

2.2 ANGSTROMS RESOLUTION STRUCTURE ANALYSIS OF TWO REFINED N-ACETYLNEURAMINYLLACTOSE-WHEAT GERM AGGLUTININ ISOLECTIN COMPLEXES

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	51.000, 73.640, 91.410
Unit cell angles	90.00, 97.91, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.172
RMSD bond length	0.018
RMSD bond angle	0.041
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.200 *
Rmerge	0.082 *
Total number of observations	10893 *
Number of reflections	17266 *
Completeness [%]	63.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.9 *	37 *	pH was adjusted to 4.9 with NaOH *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6-8 (mg/ml)
2	1	reservoir	ethanol	3-5 (%)
3	1	reservoir		6 (mM)